Janthinobacterium lividum is an aerobic, gram-negative, soil-dwelling bacterium . It is a dark purple color and is often found on the skin of amphibians especially frogs (Rebollar).
References:
Rebollar, E. A., Simonetti, S. J., Shoemaker, W. R., & Harris, R. N. (2016, April 4). Direct and Indirect Horizontal Transmission of the Antifungal Probiotic Bacterium Janthinobacterium lividum on Green Frog (Lithobates clamitans) Tadpoles. Retrieved April 5, 2017
Date collected: February 8, 2017
Methods for isolation and identification:
- Water from the top of Buffalo Creek was collected (Figure 1)
- The sample was placed onto an augar plate and kept at room temperature
- Results were taken at 24 hours and 48 hours
- A purple colony was chosen for 16s RNA sequencing by PCR amplification (Figure 2)
- The enzyme MSP1 was used to help identify the genus and species.
Figure 1. Collection Site
Figure 2. Colony collected
Results
A 1550 bp product was amplified by the PCR (Figure 3).
NCBI BLAST analysis revealed 99% match of the 16s rRNA gene of Janthinobacterium lividum (Figure 4).
Janthinobacterium lividum strain DSM 1522 16S ribosomal RNA gene, partial sequence
NR_026365.114691
| Score | Expect | Identities | Gaps | Strand | Frame |
|---|---|---|---|---|---|
| 1777 bits(962) | 0.0() | 964/965(99%) | 0/965(0%) | Plus/Plus |
Query 26 TCGAACGGCAGCACGGAGCTTGCTCTGGTGGCGAGTGGCGAACGGGTGAGTAATATATCG 85
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 34 TCGAACGGCAGCACGGAGCTTGCTCTGGTGGCGAGTGGCGAACGGGTGAGTAATATATCG 93
Query 86 GAACGTACCCTGGAGTGGGGGATAACGTAGCGAAAGTTACGCTAATACCGCATACGATCT 145
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 94 GAACGTACCCTAGAGTGGGGGATAACGTAGCGAAAGTTACGCTAATACCGCATACGATCT 153
Query 146 AAGGATGAAAGTGGGGGATCGCAAGACCTCATGCTCGTGGAGCGGCCGATATCTGATTAG 205
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 154 AAGGATGAAAGTGGGGGATCGCAAGACCTCATGCTCGTGGAGCGGCCGATATCTGATTAG 213
Query 206 CTAGTTGGTAGGGTAAAAGCCTACCAAGGCATCGATCAGTAGCTGGTCTGAGAGGACGAC 265
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 214 CTAGTTGGTAGGGTAAAAGCCTACCAAGGCATCGATCAGTAGCTGGTCTGAGAGGACGAC 273
Query 266 CAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTT 325
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 274 CAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTT 333
Query 326 TGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGAGTGAAGAAGGCCTTCGGGTT 385
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 334 TGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGAGTGAAGAAGGCCTTCGGGTT 393
Query 386 GTAAAGCTCTTTTGTCAGGGAAGAAACGGTGAGAGCTAATATCTCTTGCTAATGACGGTA 445
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 394 GTAAAGCTCTTTTGTCAGGGAAGAAACGGTGAGAGCTAATATCTCTTGCTAATGACGGTA 453
Query 446 CCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAA 505
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 454 CCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAA 513
Query 506 GCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTTTGTAAGTCTGATGTGA 565
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 514 GCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTTTGTAAGTCTGATGTGA 573
Query 566 AATCCCCGGGCTCAACCTGGGAATTGCATTGGAGACTGCAAGGCTAGAATCTGGCAGAGG 625
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 574 AATCCCCGGGCTCAACCTGGGAATTGCATTGGAGACTGCAAGGCTAGAATCTGGCAGAGG 633
Query 626 GGGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAGATATGTGGAGGAACACCGATGGCG 685
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 634 GGGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAGATATGTGGAGGAACACCGATGGCG 693
Query 686 AAGGCAGCCCCCTGGGTCAAGATTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGA 745
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 694 AAGGCAGCCCCCTGGGTCAAGATTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGA 753
Query 746 TTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCTACTAGTTGTCGGGTCTTAATTGA 805
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 754 TTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCTACTAGTTGTCGGGTCTTAATTGA 813
Query 806 CTTGGTAACGCAGCTAACGCGTGAAGTAGACCGCCTGGGGAGTACGGTCGCAAGATTAAA 865
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 814 CTTGGTAACGCAGCTAACGCGTGAAGTAGACCGCCTGGGGAGTACGGTCGCAAGATTAAA 873
Query 866 ACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCA 925
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 874 ACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCA 933
Query 926 ACGCGAAAAACCTTACCTACCCTTGACATGGCTGGAATCCCCGAGAGATTGGGGAGTGCT 985
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 934 ACGCGAAAAACCTTACCTACCCTTGACATGGCTGGAATCCCCGAGAGATTGGGGAGTGCT 993
Query 986 CGAAA 990
|||||
Sbjct 994 CGAAA 998
Figure 4. NCBI BLAST
Contributed By:
Taylor Johnson and Sarah Kamen, BIOL 250 Spring 2017