Janthinobacterium lividium is a gram negative purple pigmented bacteria that has been found by rivers, springs, and lakes all over the world.
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Date Collected: February 2, 2017
Methods for Isolation and Identification:
- Sample was taken from the river bank of the Appomattox River it was then washed and spun with sterile water, and spread on an agar plate. It was then incubated at 30 degrees Celsius for 96 hours.
- A purple colony was selected for gene sequencing by PCR amplification.
- The PCR product was digested with MspI and sequenced to identify the genus and species of the bacteria.
- MspI Digestion (Figure 4.): A 900 bp product was amplified by PCR. Upon digestion with MspI, two bands appeared under 250 bp and at 500 bp were identified.
Sequence Analysis (Figure 5): The sequenced PCR product generated 892 bases of high-quality reads that were used to identify the genus and species of the colony. NCBI BLAST analysis revealed that 99% identify with the bacteria Janthinobacterium lividium.
Contributed by: Aryana DiPippa, BIOL 250 Spring 2017, Group 5