https://drive.google.com/file/d/13Zpd_daw_VZ3-ouCzuHVurx3pHXa-uo3/view?usp=sharing
The presentation above applies the major principles of molecular biology by examining the microbiome of different animal mouths, specifically horses and dogs. My group and I took samples and plated them. These samples were incubated then critically analyzed to identify which microorganisms were present.
https://drive.google.com/file/d/1a_H1iLos5HlXWldIGb2rZWcOHq4sbYCz/view?usp=sharing
The assignment present above focused on the principle of cellular and molecular biology. My lab partner and I took, wet and dry, dirt samples from Lancer Park for PCR purification and BLAST sequencing. By plating then extracting each colony’s DNA, we were able to observe the microbial community and infer that the plates showed a difference in the microbial diversity between wet and dry soil at Buffalo Creek.
https://drive.google.com/file/d/1WkOmNOwiVaki_5uLDl0Yc-y1EOS0VtBi/view?usp=sharing
The URL posted above will direct you to a microbial contamination presentation that applies to the principle of cellular and molecular biology. In the presentation, my group and I took samples from different elevator buttons in Chichester at Longwood University and sampled them on agar plates. After the incubation process, we categorized the colonies by color and size. The results provided evidence that contamination was present and the scientific papers also supported this theory.
Reflection Portion:
The artifacts taught me how to properly analyze and apply the major principles of cell biology through experimentation and data collection. The experiment conducted in artifact one taught me how to properly observe and analyze microbial colonies. Plating, and observing microbial colonies in microbiology and cell biology is a major principle in understanding the experiment. By properly incubating and plating, I was able to observe and assume what type of bacterial species had grown on the agar plates. The skill of observing microbial communities allows me to make educated assumptions about the bacteria in an area, which can be beneficial and life-saving. This skill is one I can use forever in the science field and will sharpen as I learn more about the environment and the bacteria that live in it. The second artifact used PCR, DNA extraction and BLAST. These experimental tools help researchers, especially me, better understand what type of bacteria was collected, where is normally found, and what other bacteria share similar DNA. By learning how to use these tools, I was able to analyze my data and apply different principles of cell biology and microbiology. I am still new to these tools, therefore I do not fully understand how to work it by myself, however, I understand their importance. Once I have a better understanding, I will be able to fully utilize this skill to my benefit. For the third artifact, I was able to learn how different substances affect plant growth. By pouring different drinks, we were able to examine how photosynthesis occurred and how cells on different leaves died before others. This is important because it taught me growth on a cellular level impacts growth overall. This skill will defiantly benefit me because I can think of cell and molecular biology as a puzzle. The little pieces make the whole picture which is a major principle of cell and molecular biology.