DNA damage repair is critical for cells to maintain the correct instructions for life and avoid passing mutations to future generations. The MRN protein complex is a sensor that detects DNA damage and initiates a response to repair the damage. This complex is found in bacteria, yeast, and humans because it is so important in sensing DNA damage. In humans, the MRN complex is composed of three proteins: Mre11, Nbs1, and Rad50. Mutations in these proteins may predispose individuals to cancers, but the effects of Mre11 mutations at the cellular level are poorly understood. Using yeast as a model organism, my previous work has shown that Mre11 mutations observed in human tumors impair the function of the MRN complex. The goal of this project is to test the hypothesis that these mutations prevent physical interactions between Mre11 and its protein partners in the complex. This work will help us understand how Mre11 mutations may play a role in cancer risk. Students working on this project will gain experience in molecular biology (PCR, DNA sequencing, cloning, protein analysis) and yeast genetics, as well as skills in communication, experimental design, and analysis. Students that are highly motivated and interested in pursuing research for multiple semesters may have the opportunity to develop independent research projects aimed at understanding the molecular and cellular effects of other cancer mutations in a variety of cell types. Completion of BIOL250 is recommended, but not required. I am currently accepting 2 to 4 students for the fall semester.