PHYS 202 Tutoring Cancelled

Monday April 24, 2017

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What is Exercise Science?

Exercise science is the study of how the human body moves and works and the physical activity that affects it.

Since the time of B.C., scientists and others have always had an idea of the important of living a healthy life style in order to prevent degenerative diseases and have a good mental health. As things became to pick up towards the end of the 19th century, people began to research the difference between the lives of sedentary people versus active ones. After the conclusion of the first World War, health reports of drafted men were released. The information was alarming regarding the fitness levels of the drafted soldiers. 1 out of 3 draftees were unfit for combat and had trouble prior to bootcamp. This began the movement of implementing physical education programs into public schools. In the 1940’s,  Dr. Thomas Cureton developed the first fitness tests for various bodily functions (cardiovascular, flexibility, and muscle endurance). A decade later, Dr. Ken Cooper was recognized as “The Father of the Modern Fitness Movement” who made exercise science more of a disease prevention rather than a disease treatment. While President John F Kennedy was in office, he promoted exercise science nationally. In 1954, the American College of Sports Medicine was founded in order to research exercise-related issues. There are more and more people that try to advocate for exercise science and promoting healthy lifestyles.

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*Videos* from 2nd Semi-Annual Student Tech Showcase

Screen Shot 2017-04-13 at 2.03.14 PMWe held our second, semi-annual, Student Tech Showcase earlier this week. 
Four (4) members of the Instructional Technology Collaborator team spent approximately 15 minutes, each, connecting a DEC-supported technology to the teaching and/or learning experience. These presentations were part of the 4th tier of the ITC Badging Program and serve as the implementation component of a 3rd tier badge, Presentation Design. Each of the 4 ITCs spent the fall 2016 semester designing these presentations, working with DEC professional staff for feedback. Intended to serve as an introduction, Longwood instructors are welcome to work with the Digital Education Collaborative for further assistance on any of the technologies presented.

Videos are linked below and follow the description for the corresponding presentation. Unfortunately, we do not have a recording of Kyle’s “3D Printing in Education” session. Kyle, and Paige, will return to the team in the fall 2017 semester and can be available to work with instructors as a follow up to their sessions. Though Kristin and Michael are graduating, other ITCs will be available and are trained to assist instructors with Kahoot! and the Google Apps for Education suite.

A Kahoot! and a Half!
Kahoot! is a free, web-based platform that makes learning fun for students through its use of games. Players can join a Kahoot! game from any internet-enabled device. Professors can capitalize on existing games, or create their own, to customize the learning experience to meet specific learning objectives. Kahoot! games provide students the opportunity to interact with course content, each other, and the instructor in ways that enrich the learning environment by enhancing engagement across all domains.

Presented by: Kristin, Instructional Technology Collaborator
To view Kristin’s presentation, click here.

3D Printing in Education
Due in part to its broad applicability, 3D printing has been on the forefront of innovation in a variety of realms. This presentation will review and demonstrate the basics of 3D printing, as well as showcase sample prints previously created. Examples of 3D printing projects supporting the student learning outcomes will be provided and attendees will be encouraged to brainstorm disciplinary-specific connections.

Presented by: Kyle, Instructional Technology Collaborator
Watch Link: not available

Collaborate with Google
This session will highlight the benefits of using Google Docs as a tool for assisting collaboration on group projects as well as for individual projects that require feedback and revisions.  Topics such as how to help students create documents that are shared with other classmates as well as the instructor, in addition to the particular advantages Google Docs provides for projects that require some collaboration, will be addressed.

Presented by: Michael, Instructional Technology Collaborator
To view Michael’s presentation, click here.

Getting the most out of Canvas
Canvas has a number of built-in options to enhance the online learning environment. While these built-in options remain available to instructors, many may not know that additional options are available through Canvas apps. Working with our campus Canvas administrators, instructors are able to identify and implement pedagogically sound applications within their Canvas course(s). This session will discuss how instructors can connect an app to identified student learning outcomes, thus qualifying as pedagogically sound, ways apps can enhance the teaching and learning experience, and how to gain access to implement an app. 

Presented by: Paige, Instructional Technology Collaborator
To view Paige’s presentation, click here.

Please don’t hesitate to contact us if you have any questions or concerns as a follow up to what was covered at the Student Tech Showcase.

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KINS 205

Intro to Exercise Science has been one of the best conducted classes I have experienced in my freshman year of college. Mrs. Atkinson is an amazing person and cares not only about you as a student, but you as a person. She pushes you to strive harder and really exposes who you are. With all of her connections and network, she is able to have so many speakers come in and really give students a look at choices of careers that they could possibly enter after getting accepted into the Kinesiology program at Longwood University and graduating.

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Pseudomonas frederiksbergensis

About: Pseudomonas frederiksbergenis is a gram-negative bacteria that was originally identified in Frederiksberg, Copenhagen, Denmark at a coal gasification site. This bacteria is rod shaped, and colonies are known to be a pale yellow. Pseudomonas is known to be a species that is important for soil, food and water decomposition.

Date Collected: 2/8/2017

Methods of isolation and characterization: 

  • P. frederiksbergenis was isolated from a specific soil sample at the Environmental Education Center (EEC) located in Farmville, VA.
  • The soil sample was taken directly from Buffalo Creek, a small creek that flows into the Appomattox river.
  • Once we collected our sample we plated it onto three agar plates: direct sample, 1/10 and 1/100 dilution.
  • We observed growth at 24hrs and 48hrs and selected a specific colony to analyze that was not overlapping other colonies.
  • We ran DNA isolation and purification through Nano Drop to ensure we had a pure sample to then proceed to PCR amplification of 16s rRNA.
  • After isolating 16s rRNA of our colony we ran gel electrophoresis against MSp1 to ensure we isolated 16s rRNA to send off for sequencing.
  • Sadly we were unable to draw any conclusions from our gel due to clarity issues, never the less we went ahead and sent off our sample off to be sequenced.
  • Once sequenced, we than took our sequence and ran it through BLAST analysis which gave us a 99% match
  • Using our data analysis from BLAST as well as the color, form, and size of our colony we identified our sample to be P. frederiksbergenis.



Figure 1. Chromatogram of our Buffalo Creek soil sample, which we identified as P. frederiksbergenis.


ellie wit dirt

Figure 2. Buffalo Creek soil sample and location in which we isolated P. frederiksbergenis.


Screen Shot 2017-04-19 at 10.54.13 PM

Figure 3. Our colony of P. frederiksbergenis under a microscope. This photo highlights the pale yellow color that we used to further support our classification.



Figure 4.   The 1/100 dilution plate of the Buffalo Creek soil shows the specific colony we isolated P. frederiksbergenis.



Pseudomonas frederiksbergensis sp. nov., isolated from soil at a coal gasification site.
S. M. Andersen, K. Johnsen, J. Sørensen, P. Nielsen, C. S. Jacobsen
Int J Syst Evol Microbiol. 2000 Nov; 50(Pt 6): 1957–1964. doi: 10.1099/00207713-50-6-1957


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Janthinobacterium lividum

Janthinobacterium lividum


Janthinobacterium lividum is a gram-negative bacteria that has aerobic qualities.  It possess a pigment called violacein that gives the bacteria its dark purple color (Figure 1).

farm soil circle

Figure 1: Janthinobacterium lividum colony on agar plate

 Janthinobacterium lividum is commonly found in the skin of amphibians such as salamanders and expresses fungal and tumor fighting properties.

Date collected – February 18, 2017


Valdes, Natalia et al. (2015) “Draft genome sequence of Janthinobacterium livid strain MTR reveals its mechanism of capnophilic behavior.”

Rebiller, Eria et al. (2016) “Direct and Indirect Horizontal Transmission of the Antifungal Probiotic Bacterium Janthinobacterium livid on Green Frog (Lithobates clamitans) Tadpoles.”

Methods for Isolation and Identification

  • Soil was collected from Buffalo Creek close to a nearby farm (Figure 2)EEC siteellie collecting waterellie wit dirt

Figure 2: Collection site and the collection of surface water and bank soil

  • Sample was mixed with water, diluted, spread on agar plates, and incubated for 48 hours at room temperature
  • Colony was isolated and genomic DNA was extracted
  • PCR amplification was conducted to replicate the DNA and prepare for gel electrophoresis
  • Gel electrophoresis was used to compare the different nucleotide bases and attempt to identify the bacterial strain. However, the gel electrophoresis was unsuccessful
  • The sample was sent to be sequenced over Spring Break.  The sequences were uploaded into SnapGene Viewer, edited, and pasted into BLAST


  • The sequences were compared to the matches given by BLAST.  Janthinobacterium lividum was a 99% match with few gaps, so it was concluded that this was the colony that was isolated was a fungus (Figure 3)

gel 1 v badgel 2 v bad

Figure 3: Gel electrophoresis pictures show little success

  • The BLAST match came up with a 99% match, 1 gap, and 977 nucleotides (Figure 4).  This suggests that the bacteria isolated was almost guaranteed to be Janthinobacterium lividum


BLAST match

Figure 4: BLAST sequence versus sample sequence


Murriel Grimes, Alyssa Oppedisano, Elle Richardson

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Psudomonas Frederiksbergenesis strain dsm 13022

Pseudomonas frederiksbergensis is a Gram-negative bacteria. It was discovered by from a coal gasification site in Frederiksberg, Copenhagen, Denmark. This bacteria was cultured by Andersen S, Johnsen K, Sorensen J, Nielsen P, Jacobsen C. in 2000.


  • Andersen S, Johnsen K, Sorensen J, Nielsen P, Jacobsen C. 2000. Pseudomonas fredricksbergensis sp. nov., isolated from soil at a coal gasification site. Int. J. of Syst. and Evol. Microbiol. 50:1957-1964.
Date collected: February 8, 2017
Methods for isolation and identification:
  • Soil was taken from the Appomattox River (Figure 1), diluted by 1:10, then placed on an agar plate and isolated at room temperature for 48 hours
  • A purple colony was taken from the plate for DNA isolation and PCR amplification
  • When the PCR product was produced, it was digested with MSP1 and run through a gel
  • Once the PCR was done and the DNA was purified it was sent of for sequencing so we could determine what bacteria it was
appomattoxFigure 1: Collection site at the Appomattox River
  • The sequenced PCR product for the 16s rRNA gene generated 714 bases that were used to identify the genus and species of the bacteria colony. NCBI BLAST analysis revealed 99% identity with the bases of the 16s rRNA gene of Pseudomonas Fredricksbergenesis. There was found to be no gaps and 714 out of 716 base pairs were matched. Below shows the Blast percentages (Figure 2). Figure 3 shows the base pairs. Attatched is a more clear visual of the Blast sequence:
Score Expect Identities Gaps Strand Frame
1312 bits(710) 0.0() 714/716(99%) 0/716(0%) Plus/Plus

Figure 2: Blast data


Figure 3: The blast base pairs

Contributed by: Alyssa Oppedisano, Elle Richardson, Murriel Grimes BIOL 250 – Spring 2017, Longwood University


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COMM 392 Journal 4

Comm 200, Comm 375, and Comm 460 helped prepare me for this internship.

In Comm 200 (Intro to Comm studies), we learned about different theories. The theories we learned were theories that I could have incorporated into research projects, as part of my theoretical grounding. I have used many theories over the course of my internship including media framing, self-disclosure, social identity theory, and nonverbal communication. Prior knowledge of these theories helped me when I began to do my research.


Comm 375 (Public Relations Research) gave me a brief overview of different types of research methods. We did three projects, one survey, one content analysis, and one focus group. It was really interesting to have the opportunity to experiment with three different studies. We had a partner for the whole semester that we would write the research papers with. For the content analysis, we looked at student responses to the VP debate on Facebook and twitter, which technically helped me prepare for the content analysis I did for my internship. For the focus group we were interested in determining longwood students opinion of the campuses hook-up culture. It was interesting and I determined I really enjoyed focus groups. For the survey we wanted to find longwood students opinion of the impact of the vice presidential debate. I liked the survey the best.

Comm 460 helped me understand both quantitative and qualitative research a little more, which was helpful. I thought it would be difficult to work on my thesis and my internship research at the same, but they actually coincided nicely.

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Bacillus cereus

Bacillus cereus is a Gram-positive, rod-shaped, aerobic, motile, beta hemolytic bacterium commonly found in soil and food.


  • Been, M. De. “Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis.” Microbiology 152.10 (2006): 3035-048. Web.

Date collected: February 8, 2017

Methods for isolation and identification:

  1. Collection and Sampling of Environmental Samples at Environmental Education Center (Figure 1)
  2. Selection of bacteria from plate to be analyzed (Figure 2)
  3. Polymerase Chain Reaction to amplify 16S rRNA
  4. Restriction Enzyme Digestion & Gel Electrophoresis (Figure 3)
  5. DNA Sequencing Analysis/Identification



Figure 1. Sampling site locations in Lancer Park. The top site is Buffalo Creek and the lower site is the pond where samples were taken


Figure 2: picture of Bacillus cereus


  • MspI digestion (Figure 3): A 1,500 bp product was amplified by PCR. Upon digestion with a band at 500 bp digested and a 1000 bp undigested.

PW1 electrophoresis

Figure 3: Mspi digestion of Bacillus cereus PCR product

  • Sequence analysis (Figure 4): The sequenced PCR product generated 887 bases. NCBI BLAST analysis revealed 99% identity with 887 of the bases matching the 16s rRNA gene of Bacillus cereus (Figure 4).

PT10 sequence

  • Figure 4: The BLAST sequence match of the sample bacteria to Bacillus cereus

Contributed by: Matthew W. Bowman & Carly Carter, BIOL 250 – Spring 2017, Longwood University

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History Tutoring

History Tutoring Cancelled 4/19/17

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