Aquasprillum Arcticum Strain

Aquasprillum Arcticum is a bacteria isolated from an arctic sediment. Oxidase-positive. Usually catalase- and phosphatase-positive, Indole- and sulfatase-negative. Assigned to the genus Aquasprillum on the basis of its morphological and physiological characteristics. Usually occur in stagnant, freshwater environments. The reason we identified Aquasprillum Arcticum to be our bacteria was because it does show up in freshwater environments and its genomic sequence was  almost 97% identical to our sequence.

  • References 

Characterization of Aquaspirillum arcticum sp. nov., a New Psychrophilic Bacterium

Article in Systematic and Applied Microbiology 12(3):263-266 · December 1989
DOI: 10.1016/S0723-2020(89)80072-4

Date Collected: February 8, 2017

The study site was located behind the Environmental Educational center at Buffalo Creek and the Pond in Lancer Park near the campus of Longwood university in Farmville, Virginia.  Buffalo Creek is a 21.1-mile-long (34.0 km) tributary of the Appomattox River in Virginia. It Flows entirely through Prince Edward County, until it joins the Appomattox River at Farmville. The Pond behind the Environmental Educational center is a collection of run off from Buffalo creek and nature its self.

Methods for isolation& identification 

  •    Collected the samples at Buffalo Creek and the pond right behind lancer park. Used the tubes provided by the professor to collect the samples.  Collected  samples 6 inches deep into the water. Plates were brought back to EEC to be mixed with water and then spread onto LB agar plates, then incubated in room temperature for 48 hours.
  • A  dark purple colony (figure 2) was selected for 16S rRNA gene sequencing by PCR amplification.
  • The product in figure 2 was digested  with mspI and sequenced to identified what the genus and species of the bacteria.

 

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Figure 1. Site of collection 

Colony pic 1colony pic 2 ( pond)

Figure 2. Colony Identification 

 

  • Results

MspI digestion (figure 3) In the gel the PCR was not amplified making it hard to find good sequences. 2 bands that can be clearly identified in our gel look to be between 1,200- 1,500.

 

 

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Figure 3. Results of PCR amplification and MspI digestion

 

  • Sequence analysis(figure 4) 

The sequenced PCR product generated 475 bases of high-quality reads that were used to identify the genus and species of the colony. The chromatogram of the sequence as a PDF SKRFC_PREMIX_JF7578_46 . Blast indicated that genomic sequence was 97% identical to our sequence.

 

 

 

 

Figure 4. 16s rRNA gene of Aquasprillum arcticum 

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